SOFI’s scanning optical system enables you to analyze cells in ways that would be impossible with a flow cytometer or a camera-based imaging platform.
SOFI offers 2 excitation options plus fluorescent detection in 3 bands for up to 7 different image parameters per sample image.
Brightfield/transmitted light images provide rich morphological image detail. Variable scan profiles enable rapid acquisition of a full field of view as well as scanning regions of interest at higher resolution.
Laser-based autofocus plus an intuitive experiment setup wizard enable walk-away analysis of 96-well experiments.
Individual samples may be scanned multiple times at different image resolutions and/or different excitation intensities. Samples may be scanned before and after treatment, providing a benchmark for each sample.
With two excitation options and five fluorescence channels, SOFI has the flexibility to perform cell viability and cytotoxicity on adherent or suspension cells using a variety of popular labels.
To learn more, download our application note demonstrating viability with calcein AM and cytotoxicity using DRAQ7.
Using DRAQ5 to label nuclei, measuring additional cell parameters is easy whether the cells are live or dead.
In this app note, we used calcien AM to quantify cell viability.